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Sangon Biotech sanger sequencing
Sanger Sequencing, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+sequencing/sanger+sequencing/pmc12907642-87-6-9
Average 86 stars, based on 1 article reviews
sanger sequencing - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: mmu_circ_0012122/mmu-miR-1843-5p/Sertad2 axis: A novel regulatory pathway in rabies virus infection.
Article Snippet: .. To confirm the junction sequences of circRNAs, PCR products of divergent primers were gel purified and submitted for Sanger sequencing at Sangon Biotech (Shanghai, China). ..

Article Title: Zygote electroporation of base editor ribonucleoproteins enables efficient single-target editing but reveals limitations of multiplex base editing in sheep.
Article Snippet: Improving fecundity and growth is a major goal in sheep breeding.. Here, we established a zygote electroporation platform to deliver base editor ribonucleoproteins (BE RNPs) targeting the fecundity-associated BMPR1B(FecB) and growth-associated SOCS2 loci.. Single-target validation achieved high editing efficiencies (95.0% for BMPR1B and 65.0% for SOCS2) without compromising embryonic development in vitro, although bystander edits were prevalent.

Article Title: Functional divergence and stage-specific symbiosis of endophytic Tulasnella fungi in the endangered orchid Paphiopedilum malipoense .
Article Snippet: .. Sanger sequencing of the purified PCR products was conducted by Shanghai Sangon Biotech Co., Ltd. (China). ..

Article Title: Establishment of methods for the detection of Salmonella species by conventional and quantitative real-time PCR
Article Snippet: .. Putative positive clones were screened by PCR using M13-F/R primers and were verified by Sanger sequencing (Sangon Biotech, Shanghai, China). .. The confirmed recombinant plasmid, designated pBlue-STM1410, was extracted using a Plasmid Mini Kit (AG21001, Hunan Accurate Biotechnology Co., Ltd.) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), then converted to several copies.

Purification:

Article Title: mmu_circ_0012122/mmu-miR-1843-5p/Sertad2 axis: A novel regulatory pathway in rabies virus infection.
Article Snippet: .. To confirm the junction sequences of circRNAs, PCR products of divergent primers were gel purified and submitted for Sanger sequencing at Sangon Biotech (Shanghai, China). ..

Article Title: Functional divergence and stage-specific symbiosis of endophytic Tulasnella fungi in the endangered orchid Paphiopedilum malipoense .
Article Snippet: .. Sanger sequencing of the purified PCR products was conducted by Shanghai Sangon Biotech Co., Ltd. (China). ..

Sequencing:

Article Title: mmu_circ_0012122/mmu-miR-1843-5p/Sertad2 axis: A novel regulatory pathway in rabies virus infection.
Article Snippet: .. To confirm the junction sequences of circRNAs, PCR products of divergent primers were gel purified and submitted for Sanger sequencing at Sangon Biotech (Shanghai, China). ..

Article Title: Zygote electroporation of base editor ribonucleoproteins enables efficient single-target editing but reveals limitations of multiplex base editing in sheep.
Article Snippet: Improving fecundity and growth is a major goal in sheep breeding.. Here, we established a zygote electroporation platform to deliver base editor ribonucleoproteins (BE RNPs) targeting the fecundity-associated BMPR1B(FecB) and growth-associated SOCS2 loci.. Single-target validation achieved high editing efficiencies (95.0% for BMPR1B and 65.0% for SOCS2) without compromising embryonic development in vitro, although bystander edits were prevalent.

Article Title: Functional divergence and stage-specific symbiosis of endophytic Tulasnella fungi in the endangered orchid Paphiopedilum malipoense .
Article Snippet: .. Sanger sequencing of the purified PCR products was conducted by Shanghai Sangon Biotech Co., Ltd. (China). ..

Article Title: Establishment of methods for the detection of Salmonella species by conventional and quantitative real-time PCR
Article Snippet: .. Putative positive clones were screened by PCR using M13-F/R primers and were verified by Sanger sequencing (Sangon Biotech, Shanghai, China). .. The confirmed recombinant plasmid, designated pBlue-STM1410, was extracted using a Plasmid Mini Kit (AG21001, Hunan Accurate Biotechnology Co., Ltd.) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), then converted to several copies.

other:

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Article Title: BARD1 phase separation orchestrates a repair hub by enriching XRCC5 to drive chemoresistance in glioma.
Article Snippet: Temozolomide (TMZ) resistance is a major challenge in glioblastoma (GBM).. The role of BRCA1-associated RING domain protein 1 (BARD1), a DNA damage response protein, in GBM and its potential link to liquid-liquid phase separation (LLPS) remain unclear.. Bioinformatics analysis of TCGA, CGGA, and GEO datasets revealed that high BARD1 expression correlates with poor prognosis in GBM.

Clone Assay:

Article Title: Establishment of methods for the detection of Salmonella species by conventional and quantitative real-time PCR
Article Snippet: .. Putative positive clones were screened by PCR using M13-F/R primers and were verified by Sanger sequencing (Sangon Biotech, Shanghai, China). .. The confirmed recombinant plasmid, designated pBlue-STM1410, was extracted using a Plasmid Mini Kit (AG21001, Hunan Accurate Biotechnology Co., Ltd.) and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), then converted to several copies.

Agarose Gel Electrophoresis:

Article Title: Zygote electroporation of base editor ribonucleoproteins enables efficient single-target editing but reveals limitations of multiplex base editing in sheep.
Article Snippet: Improving fecundity and growth is a major goal in sheep breeding.. Here, we established a zygote electroporation platform to deliver base editor ribonucleoproteins (BE RNPs) targeting the fecundity-associated BMPR1B(FecB) and growth-associated SOCS2 loci.. Single-target validation achieved high editing efficiencies (95.0% for BMPR1B and 65.0% for SOCS2) without compromising embryonic development in vitro, although bystander edits were prevalent.



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